3D information can be obtained from the 2D EM data collected in a variety of ways, including single particle analysis, , helical reconstruction, electron tomography (with or without subtomogram averaging), , and 2D crystallography. The resolution of the information that can be acquired using EM is dependent on the properties of the sample, the specimen preparation method used, the technical specification of the electron microscope, and imaging parameters. Transmission electron microscopy (EM) can be used to provide structural information on a range of biological specimens from cells to macromolecules.
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